The polymerase chain reaction (PCR) is a powerful tool for detection of minute amounts of nucleic acids. Due to the exponential amplification of the target sequence, it has exquisite sensitivity. PCR (Polymerase Chain Reaction) has transformed the way molecular diagnostics are utilized for Health Monitoring of Rodent Pathogens and in obtaining disease data.
The Speed, Specificity and Exactness with which pathogens are identified by PCR enable it the most preferred method for Health Monitoring of animal.
Methodology Adopted
In PCR based Rodent Pathogen Screening, the nucleic acid both DNA & RNA is isolated from the sample (Organ, Blood, Tissue, etc.,) then amplified (after an extra reverse transcription step in the case of RNA) using highly precise primers complementary to known target pathogen sequences. References and controls are run simultaneously; internal controls monitor extraction and PCR efficiency. Amplified sequences are envisaged and documented using established protocols and instrumentation.
Rodent Pathogens Tested
The following are the Rodent Pathogens that are frequently tested at Vivo Bio Tech. We are open for providing screening services for any additional rodent pathogens as requested by the Sponsor.
| Rat | Mice | Guinea Pig | Rabbit |
| Hantaan Virus | Ectromelia Virus | Guinea Pig Adenovirus | Pasteurella spp |
| Kilham's Rat Virus (KRV) | Hantaan Virus | Guinea Pig Cytomegalovirus | Toxoplasma spp |
| Lymphocytic Choriomeningitis Virus (LCMV) | K Virus | LCMV | Bordetella bronchiseptica |
| Mouse Adenovirus types 1 and 2 | Lactic Dehydrogenase Elevating Virus (LDEV) | Sendai Virus | Ectoparasites |
| Pneumonia Virus of Mice (PVM) | Lymphocytic Choriomeningitis Virus (LCMV) | Bordetella bronchiseptica | Endoparasites |
| Rat Minute Virus (RMV) | Minute Virus of Mice (MVM) | Pasteurella spp | Enteric Protozoans |
| Rat Parvovirus (RPV) | Mouse Adenovirus types 1 and 2 |
Ectoparasites | |
| Rat Theiler Virus (RTV) | Cytomegalo Virus (MCMV) | Encephalitozoon cuniculi | |
| Sendai Virus | Mouse Hepatitis Virus (MHV) | Endoparasites |
Used For:
- Programmed screening of Animal House sentinel animals
- Ensuring that Animal Houses’ are free of the pathogens
- Early prevention of spread of these pathogens among Animal House
- Reduce human exposure to these pathogens
- Confirm disease causing agents
Transportation, Storage & Discarding
- Sample is quick freezed in Liquid Nitrogen and will be transported along with the dry ice in DNase and RNase free vials.
- The dissected samples are transported through tightly capped cryogenic vials.
- The received samples are stored in -80C Freezer
- Left over organ samples are immersed in Phenol and then discarded (A sample discarding certificate is provided to the sponsor by Vivo Bio Tech). The waste is collected by PCB authorized biomedical waste disposable agency
Turnaround Time: 5 business days
Methodologies: Conventional PCR
Normal Range: Not detected
For more information, please send your queries to – contact@vivobio.com